Daphne and the surrounding regions have been populated since from at least 9,000 BCE. European settlers eventually displaced the Native Americans. After a variety of wars and treaties the area became part of the United States in 1814. Except for a period under the flag of the Confederate States of America, Daphne and its environs have remained part of the United States until this time. From Native American, to the Spanish, French and British, the city has seen a lengthy parade of historic influences which gives Daphne its present character.
Early settlers to the region were hunter-gatherer tribes similar to those in North Alabama. A variety of Native American populations visited the area that would become Daphne including Tensaw, Choctaw, Creek and Seminole. This area came to be known as neutral ground where tribes would meet and discuss the relationships between their nations. Small groups worked together to acquire food and to provide for their families. Initially, these groups enjoyed an economy based upon hunting and scavenging but as time passed production of weapons and pottery became more advanced. During the late Woodland stage Native Americans began to practice more elaborate ritual services. Although no burial grounds are known in Daphne, they are scattered throughout nearby Baldwin County.
By 1500, the zenith of Native American culture in South Alabama, it is estimated that a community of about 5,000 lived within 50 miles of the seacoast. These peoples were among the first who met Spanish explorer Hernando de Soto in 1540. From this meeting forward, the original settlers faced a disaster from which they did not recover.
Daphne and the surrounding land shifted between English, French, Spanish and United States control.